Fibrolamellar Cancer Cells

Fibrolamellar Hepatocellular Carcinoma (FL-HCC) stem cells were isolated from ascites fluid using special culture conditions to select for cancer stem cells (CSCs) and were then transplanted into immunocompromised mice.  Tumors formed and were established as a transplantable tumor line, that can be passaged from mouse to mouse; Such a tumor line is referred to as a patient-derived xenograft (PDX) model. This PDX model is the first ever established of a human FL-HCC tumor.  Extensive characterizations of this PDX model elucidated many of its phenotypic traits including the DNAJB1-PRKACA fusion transcript, characteristic of FL-HCC tumors.  These FL-HCC tumor cells are most easily maintained ex vivo in defined culture conditions as spheroids (purified tumor cells) or organoids (mix of tumor and mesenchymal cells)..  The PDX model is unique in being highly enriched (>60%) in cancer stem cells (CSCs), a property correlating with some of the devastating properties known for this type of cancer. The initial detailed characterizations of this PDX model  was reported in Nature Communications 2015 Oct 6;6:8070. doi: 10.1038/ncomms9070.

We want to thank the Davis Family and the Fibromellar Cancer Foundation. A percentage of the proceeds will be donated to the foundation (http://fibrofoundation.org/)

Frozen Human Fibrolamellar Spheroids -- 0.5-10M cells

#35002-010 Donor Lot Tu-2010 frozen spheroids of human tumor cells isolated from a solid liver fibrolamellar hepatocellular carcinoma (FL-HCC) that has been propagated in a mouse Patient Derived Xenograft (PDX) model and contains the...

  • Price: $2,500.00

Kubota's FL-HCC Spheroid Growth Media -- 250 ml

#35101-250 Kubota's FL-HCC Spheroid Growth Media. The growth media is formulated to support the growth of the FL-HCC cancer stem cells grown as spheroids. The serum free media is made complete and is kept frozen until ready for use.

  • Price: $230.00

FL-HCC Spheroid Dissociation Media -- 30 ml

#35301-030 FL-HCC Spheroid Dissociation Media. The dissociation media is formulated to dissociate the FL-HCC spheroids into small clumps of cells for expansion of the FL-HCC cancer stem cells. Kept frozen until ready to use.

  • Price: $150.00

Spheroid Wash Buffer -- 500 ml

#35302-500 Spheroid Wash Buffer. The wash buffer is formulated to serve as a media that can be used to wash the Dissociation Media from the dissociated spheroids.

  • Price: $50.00

FL-HCC Spheroid Freezing Media -- 50 ml

#35201-050 Spheroid Freezing Media formulated to support freezing spheroids and maximizing recovery during the freeze/thaw process for >90% viability post thaw. The serum free media is made complete and is kept frozen until ready for use.

  • Price: $50.00
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PhoenixSongs Ethics Policy on Human Cells and Tissues

All stem/progenitor cells, mature cell types distributed by and used in PhoenixSongs in vitro studies come from tissues that have been rejected-fortransplant and obtained from major university hospitals or US accredited organ procurement organizations. The FL-HCC spheroids come from ascites fluid obtained through direct donation. Consent is obtained from the donor, or donor’s next of kin, and conforms to all country specific federal and local legal and ethical standards (including ICH, HIPAA, Uniform Anatomical Gift Act, National Organ Transplant Act and UK HTA requirements). From these organizations or direct donors, a copy of the donor consent, medical history, and infectious disease testing for the presence of HIV, HBV, HCV, HSV, CMV and syphilis is received and reviewed to assure that each organ may be used for research purposes. Additionally, each tissue supplier working with PhoenixSongs has in place with the source facility material transfer agreements, and review of the process by the ethics committee or Institutional Review Board (IRB) from each source facility. We review these documents at the initiation of any new donor source, and if there has been any change or revision. Before processing the tissue, it is customary to review the donor consent paperwork to assure the isolation team that the donor, or donor’s next of kin, has given consent for use of the tissue for research purposes. These reports and all other materials pertaining to the donor, the donated organ, and its processing are maintained in a physically secured location, with access available only to authorized persons. The records are stored in a manner to comply with HIPAA regulations; the records are de-identified. Any reference to the donor’s identity is removed.